These 3M Attest Self Contained Biological Indicators are for use with gravity displacement steam sterilizers at 270°F/132°C. Chemical indicator on the label changes from rose to brown when processed. After sterilization, the vial is "crushed" to join the growth media with the processed spore strip.The BI is incubated for 24 hours for a visual color change readout. A color change to yellow indicates surviving spores and a positive result.

• A spore burden beyond the average bioburden (germ count) provides an extra margin of safety.
• Optimized media promote rapid growth of spores.
• Definitive color change of the media indicates sterilization process ineffectiveness.
• Result verification time of 24 hours